ANALISIS INTERAKSI SENYAWA SAPONIN DENGAN PROTEIN TERLIBAT DALAM PERTAHANAN TUMBUHAN TERHADAP PATOGEN SECARA IN SILICO
DOI:
https://doi.org/10.36709/ampibi.v8i4.32Keywords:
In Silico, Protein CATHB3, Protein dVPE, Senyawa SaponinAbstract
Penelitian ini bertujuan untuk mengeksplorasi potensi senyawa saponin sebagai antimikroba untuk patogen tumbuhan dengan memanfaatkan pendekatan In Silico. Identifikasi senyawa dilakukan melalui berbagai webserver database seperti Phubchem NCBI, PASS Online, PharmMapper, Swiss Target Prediction, SuperPred, STRING DB, dan SAVES. Hasil analisis menunjukkan bahwa terdapat potensi antimikroba pada senyawa Saponin dengan nilai Pa>0,3 secara In Silico. Ditemukan juga adanya interaksi protein-protein yang terlibat dalam melawan patogen tumbuhan seperti CATHB3 dan dVPE, sedangkan Pemodelan dan validasi struktur kedua protein tersebut menunjukkan bahwa terdapat nilai validitas struktur protein yang tinggi sebesar 94-95% (ERRAT) dengan nilai residu yang sangat rendah sebesar 0.2-0.3% (Ramachandran Plot) di daerah yang tidak diinginkan (Residues in disallowed regions) semakin rendah nilai residu maka semakin tinggi nilai kecocokan struktur protein yang dianalisis. Penelitian ini diharapkan dapat menjadi dasar untuk penelitian lebih lanjut mengenai interaksi antara senyawa saponin dengan protein-protein tersebut melalui metode moleculer docking sehingga membuka peluang untuk inovasi dalam program perlindungan tanaman terhadap patogen pada penelitian selanjutnya.
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